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fastquant rt super mix kit  (tiangen biotech co)


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    Structured Review

    tiangen biotech co fastquant rt super mix kit
    Fastquant Rt Super Mix Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fastquant+rt+super+mix+kit/FastQuant+RT+Super+Mix/pmc12733306-59-23-28
    Average 99 stars, based on 36 article reviews
    fastquant rt super mix kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Identification and Characterization of the Efbzip Gene Family in Erianthus fulvus and Exploration of Functional Genes Involved in Sucrose Metabolism
    Article Snippet: .. TRIzol reagent (Tiangen, Beijing, China) was employed to extract total RNA, and complementary DNA (cDNA) synthesis was performed from this RNA using the FastQuant RT Super Mix Kit (Tiangen, Beijing, China). ..

    Article Title: Investigating the mechanisms of flucarbazone-sodium resistance in Bromus japonicus Thunb
    Article Snippet: Background Bromus japonicus Thunb. is a hazardous weed in wheat fields in China.. One B. japonicus population, HB-1, which was collected in Hebei Province, survived field rate flucarbazone-sodium exposure.. Method To investigate the mechanism of flucarbazone-sodium resistance in the HB-1 population, the herbicide target als gene was isolated, and the in vitro ALS activity was tested.

    Extraction:

    Article Title: Genome-wide identification of ADF gene family in Chinese cabbage (Brassica rapa L. ssp. pekinensis) and functional characterization of BrADF11 under heat stress.
    Article Snippet: Actin depolymerizing factors (ADFs), which function as essential actin-binding proteins (ABPs), are fundamental to plant growth, development, and stress responses by depolymerizing or severing actin filaments.. However, research on the ADF gene family in Chinese cabbage remains relatively scarce.. In Chinese cabbage, we identified 18 BrADF genes unevenly distributed across eight chromosomes.

    Reverse Transcription:

    Article Title: Genome-wide identification of ADF gene family in Chinese cabbage (Brassica rapa L. ssp. pekinensis) and functional characterization of BrADF11 under heat stress.
    Article Snippet: Actin depolymerizing factors (ADFs), which function as essential actin-binding proteins (ABPs), are fundamental to plant growth, development, and stress responses by depolymerizing or severing actin filaments.. However, research on the ADF gene family in Chinese cabbage remains relatively scarce.. In Chinese cabbage, we identified 18 BrADF genes unevenly distributed across eight chromosomes.

    Synthesized:

    Article Title: ArNAC148 induces Acer rubrum leaf senescence by activating the transcription of the ABA receptor gene ArPYR13.
    Article Snippet: Acer rubrum, an ornamental tree known for its stunning autumn colors, has an elusive molecular mechanism that governs its leaf senescence.. We performed the genome-wide analysis of NAC transcription factor genes and PYRABACTIN RESISTANCE1-LIKE (PYLs) and found that ArNAC148 and ArPYL13 were significantly upregulated in senescing leaves as compared to mature leaves.. Subcellular localization studies confirmed the nuclear localization of ArNAC148 and the cytoplasmic localization of ArPYL13.

    Article Title: The crotonylated and succinylated proteins of jujube involved in phytoplasma-stress responses.
    Article Snippet: DNA and RNA concentration and purity were checked by a NanoDrop 2000 spectrophotometer. .. First strand cDNA was synthesized by 500 ng of the total RNA with a FastQuant RT Super Mix Kit (TIANGEN). cDNA was diluted tenfold and then used as the template for next study. ..

    Article Title: The ZjMYB44-ZjPOD51 module enhances jujube defense response against phytoplasma by upregulating lignin biosynthesis
    Article Snippet: .. The RNAprep Pure Plant plus Kit (Tiangen, China) was used for RNA isolation, then cDNA was synthesized using FastQuant RT Super Mix Kit (Tiangen, China). ..

    Article Title: The peptidase DA1 cleaves and destabilizes WUSCHEL to control shoot apical meristem size
    Article Snippet: The sputter-coated samples were ready for observation and imaging using scanning electron microscope (Hitachi S-3000N). .. 6-d-old aboveground part of Col-0 and da1-1 were ground in liquid nitrogen, and the RNAprep pure kit was used to extract total RNA following the manufacturer’s instructions (TIANGEN, Cat. No: DP439-H). cDNAs were synthesized using the FastQuant RT Super Mix kit (TIANGEN, Cat. No: KR108). qPCR reactions were assayed on a Lightcycler 480 machine (Roche Applied Science, USA) using 2×SYBR Green supermix kit (Vazyme, China). ..

    Article Title: The crotonylated and succinylated proteins of jujube involved in phytoplasma-stress responses
    Article Snippet: DNA and RNA concentration and purity were checked by a NanoDrop 2000 spectrophotometer. .. First strand cDNA was synthesized by 500 ng of the total RNA with a FastQuant RT Super Mix Kit (TIANGEN). cDNA was diluted tenfold and then used as the template for next study. ..

    Purification:

    Article Title: Investigating the mechanisms of flucarbazone-sodium resistance in Bromus japonicus Thunb
    Article Snippet: Background Bromus japonicus Thunb. is a hazardous weed in wheat fields in China.. One B. japonicus population, HB-1, which was collected in Hebei Province, survived field rate flucarbazone-sodium exposure.. Method To investigate the mechanism of flucarbazone-sodium resistance in the HB-1 population, the herbicide target als gene was isolated, and the in vitro ALS activity was tested.

    Quantitative RT-PCR:

    Article Title: Investigating the mechanisms of flucarbazone-sodium resistance in Bromus japonicus Thunb
    Article Snippet: Background Bromus japonicus Thunb. is a hazardous weed in wheat fields in China.. One B. japonicus population, HB-1, which was collected in Hebei Province, survived field rate flucarbazone-sodium exposure.. Method To investigate the mechanism of flucarbazone-sodium resistance in the HB-1 population, the herbicide target als gene was isolated, and the in vitro ALS activity was tested.

    SYBR Green Assay:

    Article Title: Investigating the mechanisms of flucarbazone-sodium resistance in Bromus japonicus Thunb
    Article Snippet: Background Bromus japonicus Thunb. is a hazardous weed in wheat fields in China.. One B. japonicus population, HB-1, which was collected in Hebei Province, survived field rate flucarbazone-sodium exposure.. Method To investigate the mechanism of flucarbazone-sodium resistance in the HB-1 population, the herbicide target als gene was isolated, and the in vitro ALS activity was tested.

    Isolation:

    Article Title: The ZjMYB44-ZjPOD51 module enhances jujube defense response against phytoplasma by upregulating lignin biosynthesis
    Article Snippet: .. The RNAprep Pure Plant plus Kit (Tiangen, China) was used for RNA isolation, then cDNA was synthesized using FastQuant RT Super Mix Kit (Tiangen, China). ..

    Real-time Polymerase Chain Reaction:

    Article Title: The peptidase DA1 cleaves and destabilizes WUSCHEL to control shoot apical meristem size
    Article Snippet: The sputter-coated samples were ready for observation and imaging using scanning electron microscope (Hitachi S-3000N). .. 6-d-old aboveground part of Col-0 and da1-1 were ground in liquid nitrogen, and the RNAprep pure kit was used to extract total RNA following the manufacturer’s instructions (TIANGEN, Cat. No: DP439-H). cDNAs were synthesized using the FastQuant RT Super Mix kit (TIANGEN, Cat. No: KR108). qPCR reactions were assayed on a Lightcycler 480 machine (Roche Applied Science, USA) using 2×SYBR Green supermix kit (Vazyme, China). ..



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